A convenient, simple, specific, accurate, precise, rapid, inexpensive isocratic reversed phase-high performance liquid chromatography (RP-HPLC) method was developed and validated for the quantitative determination of mebeverine HCl in pharmaceutical tablet dosage forms. RP-HPLC method was developed by using welchrom C18 Column (4.6 X 250 mm, 5µm), Shimadzu LC-20AT prominence liquid chromatograph. The mobile phase composed of 10 mM phosphate buffer (pH-3.0, adjusted with triethylamine): acetonitrile (50:50 v/v). The flow rate was set to 1.0 ml.min-1 with the responses measured at 261 nm using shimadzu SPD-20A Prominence UV-Vis detector. The retention time of mebeverine HCl was found to be 4.683 minutes. Linearity was established for mebeverine HCl in the range of 2-10 µg.ml-1 with correlation coefficient 0.9999. The LOD and the LOQ were found to be 0.0653 μg.ml-1 and 0.1980 μg.ml-1 respectively. The amount of mebeverine HCl present in the formulation was found to be 99.90%. The validation of the developed method was carried out for specificity, linearity, precision, accuracy, robustness, limit of detection, limit of quantitation. The developed method can be used for routine quality control analysis of mebeverine HCl in pharmaceutical tablet dosage form.
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