A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of itopride hydrochloride and lansoprazole in synthetic mixture using Simultaneous equation method. The absorbance was measured at 258.00 nm for itopride hydrochloride and 284.00 nm for lansoprazole and calibration curves were plotted as absorbance versus concentration, respectively. The method was found to be linear (r2>0.9985) in the range of 5-25 μg/ml for itopride hydrochloride at 258.00 nm. The linear correlation was obtained (r2>0.9985) in the range of 5-25 μg/ml for lansoprazole at 285.00 nm. The limit of determination (LOD) was 0.233μg/ml and 0.706μg/ml for itopride hydrochloride and lansoprazole respectively. The limit of quantification (LOQ) was 0.162 μg/ml and 0.491 μg/ml for itopride hydrochloride and lansoprazole respectively. The accuracy of these methodswas evaluated by recovery studies and good recovery result was obtained greater than 99%.The method was successfully applied for simultaneous determination of itopride hydrochloride and lansoprazole in binary mixture.
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