The simple, accurate and precise absorption correction method has been developed for the simultaneous estimation of amitriptyline hydrochloride and methylcobalamin in combined tablet dosage form. The method utilizes distilled water as solvent and λmax of amitriptyline hydrochloride and methylcobalamin selected for analysis were found to be 239 nm and 351 nm respectively. The method was validated as per international conference on harmonization (ICH) guidelines. The linearity range lies between 20-60 µg/ml (R2 0.9998) for amitrityline hydrochloride and 3-9 µg/ml (R2 0.9990) for methylcobalamin. The accuracy and precision were determined and found to comply with ICH guidelines. The method showed good reproducibility and recovery with %RSD in desired range. The proposed method can be applied for routine analysis of both drugs.
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