The present work describes development and validation of stability indicating RP-HPLC method for simultaneous estimation of citicholine and piracetam in combined dosage form. The chromatographic separation was obtained by using a mobile phase comprised of phosphate buffer (pH-4.8) and methanol in the ratio 90:10 v/v using hyperchrome ODS C18 column (250 X 4.6 mm) with UV detection at 210 nm at a flow rate of 1 ml/min.The Rt was found to be 2.9 and 5.1 min for citicholine and piracetam respectively. The linearity of proposed method was tested in the range of 10-50 μg/ml for citicholine and 16–80 μg/ml for piracetam with correlation coefficients 0.998 and 0.999 respectively. The accuracy of the proposed method was determined by recovery studies and found to be 99.32% for citicholine and 99.28% for piracetam. The forced degradation study was carried out and degradation peaks are well resolved from the main peaks. The proposed validated stability indicating HPLC method for the simultaneous estimation of citicholine and piracetam in tablet dosage form was accurate, precise, linear, economic and robust. The developed method can be routinely adopted for analysis of citicholine and piracetam in the formulation.
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