Two simple, accurate and precise UV Spectroscopic methods were developed for the simultaneous estimation of escitalopram oxalate (ESO) and bupropion hydrochloride (BUP) in their synthetic mixture. For First order derivative spectrophotometric method 251 nm was selected for the estimation of escitalopram oxalate (ZCP for bupropion hydrochloride) and 237 nm was selected for the estimation of bupropion hydrochloride (ZCP for escitalopram oxalate). For dual wavelength method 235.6 nm and 240 nm were selected for the determination of bupropion hydrochloride, similarly 245 nm and 257.6 nm were selected for the determination of escitalopram oxalate. The linearity range was found to be 10-30 µg/ml for both ESO and BUP in first order derivative method and 5-25 µg/ml for both ESO and BUP in dual wavelength method. For first order derivative method correlation coefficient were found to be 0.9993 and 0.9994 for ESO and BUP respectively and the assay value was found to be 100.53% and 99.73% respectively, similarly for dual wavelength method correlation coefficient were found to be 0.9991 and 0.9994 for ESO and BUP respectively and the assay vale was found to be 99.1% and 99.4% respectively. The methods were validated as per the ICH guidelines. The proposed validated methods were successfully used for the quantitative analysis of synthetic mixture.
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