A simple, rapid, accurate, precise and economic RP-HPLC method was developed for the estimation of pharmaceutical dosage forms using c18 column (250×4.6 mm i.d; 5µm) with mobile phase comprising of methanol and phosphate buffer in ratio of 30:70 (% v/v) triethylamine as ion pair reagent. The flow rate 1.2 ml/min and the detection was carried out at 221 nm with variable wavelength detector. The retention time (t r) of calcium dobesilate and docusate sodium was found to be 1.93 and 2.20 min, respectively. The method has permitted linear response in concentration range of 5-25 µm/ml for and limit of detection and limit of quantification of calcium dobesilate and docusate sodium was found to be 4.60 and 1.29, respectively. The percentage recovery was found to be 99.66 to101.0 and % RSD of system precision and method precision of calcium dobesilate and docusate sodium was found to be 1.038, 1.29 and 1.03, 1.70 respectively. The method was validated as per ICH guidelines for various parameters such as accuracy, precision, linearity, robustness.
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