Bacopa monnieri is traditional ayurvedic herb that improves intellectual and enhance brain memory which is generally co-cultivated with rice paddy and rice paddy is sprayed with number of organochloro and organophosphate pesticides. A specific, accurate and precise Gas chromatographic method has been developed and validated for detection and quantification of pesticides namely hexachlorobenzene, chlorpyrifos and carbophenothion in Bacopa monnieri. The method development was achieved by using Restek RTX-5 (30 m, 0.25 mm i.d., 0.25 μm) along with electron capture detector using acetone as a solvent with flow rate 5.3 ml/min. The retention time of hexachlorobenzene, chlorpyrifos and carbopenothion were found to be 12.8 min, 17.5 min and 23.5 min respectively. The method was validated as per ICH guidelines. The linearity and range was found to be 0.050 – 0.100 μg/ml for each three pesticides. The co-relation coefficient was found to be 0.9956, 0.9957 and 0.9972 respectively. % RSD of repeatability, intraday and interday precision was found to be less than 2%. % RSD for robustness parameters (flow rate change and Ramp temp change) was found to be less than 2%. The % recovery of hexachlorobenzene, chlorpyrifos and carbopenothion at different levels were found in the range of 86.25% - 91.90%, 88.62% -97.08% and 88.87% - 91.10% respectively. So the developed method was precise, accurate and robust. The developed method was simple, specific, economic and can be used for simultaneous estimation of hexachloro-benzene, chlorpyrifos and carbophenothion in Bacopa monnieri tablet dosage form.
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