The present manuscript describes new, simple, accurate and precise high performance thin layer chromatography method for the simultaneous determination of mebeverine hydrochloride and chlordiazepoxide in combined pharmaceutical dosage form. Chromatographic separation of the drugs was performed on aluminium plates pre coated with silica gel 60 F254 as the stationary phase and the solvent system consisted of Diethyl ether: Methanol: Ethyl acetate: Triethylamine (7:2:1:0.1, v/v/v/v). Densitometric evaluation of the separated zones was performed at 273 nm. The two drugs were satisfactorily resolved with Rf values 0.35 and 0.63 for mebeverine hydrochloride and chlordiazepoxide, respectively. The linear regression data for the calibration plots showed good relationship with r2 = 0.9990 from 1350-8100 ng/spot for mebeverine hydrochloride and r2 = 0.9995 from 50-300 ng/spot for chlordiazepoxide. The methods were validated for precision, accuracy and recovery. The percentage recovery for mebeverine hydrochloride was found to be 99.10 –100.76% and 99.90 – 100.72% for chlordiazepoxide. The limits of detection and quantification were 148.20 and 5.78 ng/spot per spot for mebeverine hydrochloride and 449.11 and 17.52 ng/spot per spot for chlordiazepoxide, respectively.
Loading....