Easy, sensitive, cost effective and consistent uv-spectrophotometric method has been developed for simultaneous estimation of quercetin and curcumin in topical gel formulation and validated. Quercetin and curcumin shows absorption maxima at 371 nm and 424 nm respectively. The developed method has followed linearity in the range of 2-7 μg/ml for quercetin and 1-6 μg/ml for curcumin. The value of correlation coefficient was 0.9993 and 0.999 respectively. Satisfactory values of percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (100 to 105%) showed accuracy of the method. LOD and LOQ were calculated as 0.0357 μg/ml and 0.1086 μg/ml for quercetin and 0.1133 μg/ml and 0.3434 μg/ml for curcumin respectively. The developed method can be used for routine estimation of quercetin and curcumin in pharmaceutical topical gel.
Loading....