Eugenol shows various biological activities such as antioxidant, anti-inflammatory, antibacterial, antifungal and antiviral. The present study describes the degradation behaviour of eugenol under different stress conditions (hydrolysis, oxidation, photolysis and thermal decomposition) in order to establish a validated stability-indicating high-performance liquid chromatography method. The method which was developed have the acetonitrile and phosphate buffer (85:15) as a mobile phase and the detection was carried out at 280 nm, at flow rate of 1.2 ml/min. The retention time of eugenol was 7 minutes. Validation of the method was also performed in which linearity, precision, limit of detection, limit of quantitation and robustness was performed.
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