Reverse phase high performance liquid chromatographic (RPHPLC) method was developed for the simultaneous estimation of naltrexone and bupropion in combined dosage form. The separation was achieved by C18 Column (250 x 4.6 mm, 5 µm Particle Size) and the mobile phase consisting of 0.05 M potassium dihydrogen phosphate (pH adjusted to 4.5±0.1): methanol (30:70 (V/V)). Detection was carried out at 224 nm. Retention time of naltrexone and bupropion was found to be 6.81 minutes and 4.26 minutes respectively. This method has been validated for linearity, accuracy and precision. Linearity for naltrexone was found in range of 2 to 6 mcg/ml and bupropion was found in range of 22.5 to 67.5mcg/ml. The coefficient correlation of naltrexone and bupropion was found to be 0.9991 and 0.9986 respectively. The percentage recoveries for naltrexone and bupropion were found to be in range of 99.52 to 100.31% and 100.11 to 100.70% respectively. In this method the first order derivative of UV spectrometry method was found to be simple, accurate, fast, precise and reproducible. The first derivative values measured at 210 nm for naltrexone and 264 nm for bupropion. The linearity for naltrexone and bupropion were found in the range of 2 to 6 mcg/ml and 22.5 to 67.5 mcg/ml respectively. The coefficient correlation of naltrexone and bupropion was found to be 0.9998 and 0.9994 respectively. The percentage recoveries obtained for naltrexone and bupropion were found to be in range of 99.38 to 99.92% and 99.26 to 99.94% respectively. Developed method was found to be accurate, precise and rapid for simultaneous estimation of naltrexone and bupropion.
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