Aim of this research was to develop a simple, precise, accurate and reproducible first order derivative method spectrophotometry method for estimation of ivabradine HCl and atenolol in combination of synthetic mixture in 1:10 ratios and validate it as per ICH guidelines. The method is based on first order UV derivative spectrophotometry. Methanol was selected as a solvent for estimation of ivabradine HCl at ZCP of atenolol 299.30 nm and for atenolol at ZCP of ivabradine HCl 287.00 nm in linearity range 2-10 µg/ml and 20-100 µg/ml respectively. The correlation coefficient (r2) for ivabradine HCl was 0.9999 and for atenolol 0.9994. The mean % recovery was found to be in range of 100.23%-101.00% for ivabradine HCl and 100.17%-101.41% for atenolol. The result of analysis has been validated statistically and recovery studies recommended for routine since it is rapid, simple, accurate, sensitive.
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