A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of saxagliptin hydrochloride and glibenclamide in synthetic mixture using first order derivative zero-crossing method. Saxagliptin hydrochloride showed zero crossing point at 315.00 nm while glibenclamide showed zero crossing point at 229.40 nm. The dA/dλ was measured at 229.40 nm for saxagliptin hydrochloride and 315.00 nm for glibenclamide and calibration curves were plotted as dA/dλ versus concentration, respectively. The method was found to be linear (r2>0.9995) in the range of 5-25 μg/ml for saxagliptin hydrochloride at 229.40 nm. The linear correlation was obtained (r2>0.9994) in the range of 5-25 μg/ml for glibenclamide at 315.00 nm. The limit of determination was 0.243μg/ml and 0.317μg/ml for saxagliptin hydrochloride and glibenclamide, respectively. The limit of quantification was 0.738 μg/ml and 0.960 μg/ml for saxagliptin hydrochloride and glibenclamide respectively. The accuracy of these method were evaluated by recovery studies and good recovery result were obtained greater than 99% shows first order derivation zero crossing. The method was successfully applied for simultaneous determination of saxagliptin hydrochloride and glibenclamide in binary mixture.
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