A simple, efficient, precise and accurate spectroscopic method has been developed and validated for quantitative estimation of cycloserine in bulk and pharmaceutical dosage form. Cycloserine standard solution was scanned in the UV rang (400-200 nm) in a 1 cm quartz cell in a double beam UV spectrophotometer. The max of cycloserine was 216.6 nm. The method obeys beers law in the concentration range from 5-25 μg/ml. The correlation coefficient was found to be 0.9996 and regression of the curve was found y = 0.0302x + 0.0128 with excellent recovery 99-101%. Limit of detection and limit of quantitation were found to be 0.24541 mg/ml and 0.79894 mg/ml respectively. The method was validated for several parameters like accuracy, precision as per ICH guidelines. Value of % RSD and % recovery was found satisfactory, hence that the proposed method is precise, accurate and economical hence can be routine analysis.
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