A reverse phase liquid chromatography (LC) method was developed and validated for simultaneous estimation of mupirocin and beclomethasone dipropionate in ointment Formulation. The isocratic LC analysis was performed on Phenomenex Gemini ODS C18 column (200 mm x 4.6 mm, 5μ) using mobile phase composed of Methanol: Buffer pH 4 (65:35, v/v) at a flow rate of 1.0 ml/min. Quantitation was performed using UV detector at 215 nm. The retention times were found to be 3.36 min for mupirocin and 6.02 min for beclomethasone dipropionate. The analytical method was validated according to ICH guidelines. The linearity was observed in the range of 40-120 and 0.5-1.5 μg/ml with correlation coefficient, r=0.996 and 0.997 for mupirocin and beclomethasone dipropionate respectively. The accuracy (%recovery) was found to be 99.45-99.86% for mupirocin and 100.10-100.39% for beclomethasone dipropionate. The relative standard deviation values for repeatability and intermediate precision studies were less than 2%. The method was successfully applied for market sample analysis and mean percentage assay values were 98.37±0.49 and 97.19±1.379 for mupirocin and beclomethasone dipropionate respectively. The present method is precise and accurate and can be used for the routine estimation of mupirocin and beclomethasone dipropionate in ointment formulation.
Loading....