A reverse phase liquid chromatography (LC) method was developed and validated for simultaneous estimation of meclizine and folic acid in tablet dosage form. The isocratic LC analysis was performed on phenomenex gemini ODS C18 column (200 mm x 4.6 mm, 5μ) using mobile phase composed of buffer:methanol pH 3.5 adjusted by phosphoric acid (65:30, v/v) at a flow rate of 1.0 ml/min. Quantitation was performed using UV detector at 210 nm. The retention times were found to be 3.760 min for meclizine and 6.133 min for folic acid. The analytical method was validated according to ICH guidelines. The linearity was observed in the range of 12.5-37.5 and 1.25-3.75 μg/ml with correlation coefficient, r=0.997 and 0.998 for meclizine and folic acid respectively. The accuracy (%recovery) was found to be 98.73 - 100.47 % for meclizine and 98.83 – 101.07 % for folic acid. The relative standard deviation values for repeatability and intermediate precision studies were less than 2%. The method was successfully applied for market sample analysis and mean percentage assay values were 97.97 and 100.24 for meclizine and folic acid respectively. The present method is precise and accurate and can be used for the routine estimation of meclizine and folic acid in tablet dosage form.
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