Simple, rapid, sensitive, economic and reproducible methods were developed for the estimation of Atazanavir and Cobicistat in synthetic mixture. The developed method were validated in accordance with ICH Q2 guidelines. Method A is first order derivative spectroscopic method was performed at detection wavelength 319.85 nm for atazanavir and 248.25 nm for cobicistat. Method B is dual wavelength spectroscopic method was performed at detection wavelength 226.30 nm and 247.15 nm for atazanavir and 238.46 nm and 258.81 nm for cobicistat. Methanol was used as solvent in the both methods. In both the methods drugs shows linearity in the concentration range of 5-30 µg/ml for atazanavir and 15-90 µg/ml for cobicistat. Results of analysis for the both the methods were validated statistically and by recovery studies. The developed methods could be used for routine analysis of atazanavir and cobicistat in synthetic mixture due to simplicity, ease of operation and short time required for quantification.
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