The present work describes two simple, precise and economical UV methods have been developed for the estimation of esomeprazole magnesium trihydrate in bulk and pharmaceutical dosage form. Distilled water was selected as common solvent for estimation of esomeprazole. First order derivative spectra showed sharp peak at 300.6 nm and area under curve method involves the calculation of integrated value of absorbance with respect to the wavelength between two selected wavelength 290-310 nm respectively. The linearity range was found to be 3-21 μg/ml in distilled water for both methods. The regression equation were found to be y = -0.0001x - 0.0002 (r2 = 0.9986) for first order derivative method and y = 0.103x + 0.1355 (r2 = 0.9989) for AUC method. Intraday and interday precision, % RSD for esomeprazole was found to be satisfactory. The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization (ICH). All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount. As economical solvent was used, these methods were used for routine analysis of esomeprazole in bulk and pharmaceutical formulation.
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