A simple, rapid, economic, sensitive and precise RP-HPLC method has been developed for the simultaneous estimation of ebastine and phenylephrine HCl in tablet. Literature review was done for this combination and there is no reported RP-HPLC method available for estimation of both drugs in combination. An isocratic separation was carried out using C18 column (250×4.6 mm, 5 m particle size) and the mobile phase consisting of 0.02M KH2PO4 Buffer (pH 4.0 (±0.05) is adjusted with using orthophosphoric acid) and methanol in the proportion of 70:30 (v/v). Quantification carried out at a wavelength of 272 nm. Retention time of ebastine and phenylephrine HCl was found to be 5.847 and 3.600 min respectively. The % assay of ebastine and phenylephrine HCl were found to be in range of 99.86-99.95 % and 99.89-99.93 %. The limits of detection and quantification were found to be 0.235, 0.509 μg/ml and 0.71, 1.54 μg/ml respectively.
Loading....