The main aim of this review is to provide the overview on estimation of anticancer drugs by various high performance liquid chromatographic methods. In present scenario, the most common analytical method employed for estimation of drugs is reverse phase high pressure liquid chromatography (RP-HPLC) because of its high sensitivity and speed. Analytical method development and validation is an important step in the drug discovery process. The principle that includes in the HPLC is separation of the components based on their relative affinities.
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