High performance liquid chromatography is at present one of the most sophisticated tool of the analysis. The estimation of dasatinib and lenvatinib was done by RP-HPLC. The phosphate buffer was pH 3.0 and the mobile phase was optimized which consists of methanol: phosphate buffer mixed in the ratio of 70:30 % v/v. Inertsil ODS C18 column (4.6 x 150 mm, 5 m) or equivalent chemically bonded to porous silica particles was used as stationary phase. The detection was carried out using PDA detector at 260 nm. The solutions were chromatographed at a constant flow rate of 0.8 ml/min. The linearity range of dasatinib and lenvatinib were found to be from 100-500 g/ml of dasatinib and 1-5 g/ml of lenvatinib. Linear regression coefficient was not more than 0.999. The values of % RSD are less than 2% indicating accuracy and precision of the method. The percentage recovery varies from 98-102% of dasatinib and lenvatinib. LOD and LOQ were found to be within limit.
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