A new, precise and economical spectrophotometric method has been developed and validated for the determination of lamivudine in bulk drugs. This method is based on the reaction of ninhydrin with primary amine present in the lamivudine in the presence of ammonium molybdate to form a Ruhemann’s purple product which was determined spectrophotometrically at 566.80 nm. The effects of variables such as temperature, heating time, concentration of colour producing reagent and stability of colour were investigated to optimize the procedure. This reaction proceeds quantitatively at 85±1°C in 15 min. Beer-Lambert’s law is obeyed in the concentration range of 6-20 μg/ml and is described by the regression equation Y = 0.0661x with a regression coefficient (r2) 0.9994. For lamivudine, the value of molar absorptivity and Sandell’s sensitivity are 15.486x103 L/mol/cm and 0.01486 μg/cm2 respectively. The LOD and LOQ are found to be 0.7635 and 2.313 μg/ml, respectively. The statistically validated results indicate that the proposed method has good sensitivity, accuracy, precision and stability. The method is green and economic as it does not involve any organic solvents.
Loading....