Herbal formulations are popular in worldwide due to safer and not causing any toxicity. Recently there have been reported herbal medicines adulterated with undeclared synthetic drugs. A simple, specific, accurate and isocratic RP-HPLC method was developed and subsequently validated for determination of adulterant. Separation was achieved with a Phenomax Luna CÃ?¬18 (250 Ã?â?? 4.5 mm) 5 Ã?µm column with a mobile phase comprising phosphate buffer: Acetonitrile pH4 adjusted with 0.1% ortho phosphoric acid in the volume ratio of 45:55% v/v was developed. The detection was carried out using a photo diode array detector set at a wavelength of 254 nm. The method was validated as per ICH guideline demonstrating the accuracy and precision within corresponding linearity range of 10 Ã?µg/ml to 60 Ã?µg/ml and get the correlation coefficient of paracetamol, diclofenac sodium and aceclofenac were found to be 0.995, 0.997 and 0.995, respectively. The developed method was validated with respect to linearity, accuracy, precision, limit of detection and quantification and robustness. The method can be used for quality control of herbal formulation.
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