The simple, rapid, sensitive, precise and specific high-performance thin layer chromatographic (HPTLC) method for the determination of teneligliptin hydrobromide in bulk drug and pharmaceutical dosage form was developed and validated. The concentration range of 500-3000 ng/spot with coefficient of correlation (r2) of 0. 987. For HPTLC the method employed aluminium plates precoated with silica gel G60 F254 as the stationary phase. The solvent system consisted of the chloroform:methanol:glacial acetic acid in the proportion of 8.5:1.5:0.05, v/v/v. This solvent system was found to give compact spots for the teneligliptin HBr with Rf value 0.41 ± 0.01. Densitometric analysis of the teneligliptin HBr was carried out in the absorbance mode at 254 nm. The developed method was validated as per the ICH guidelines. Statistical analysis proved that method is repeatable and specific for the estimation of the said drug. These methods can be adopted in routine assay analysis of the teneligliptin HBr in bulk or tablet dosage form.
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