Chromatographic studies were carried out on ethanolic stem extract of Ventilago maderspatana Gaertn belonging to the family Rhamnaceae. It is an important and well known plant in the Indian system of medicine. Stem samples of V. maderspatana was subjected to maceration by using distilled water and ethanol. Ethanolic extract was prepared from the stem, of Ventilago maderspatana. Preliminary phytochemical analysis was carried for the extract. Extract was found to contain different phytoconstituents such as alkaloids, glycosides, steroids and sterols, saponins, Flavonoids, carbohydrates and tri- terpenoids and showed the absence of amino acids, proteins and acidic compounds. Fractions were prepared according to polarity and subjected to TLC and HPTLC analysis.
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