The present article describes about the development of zero order UV spectrophotometric method for the quantitative determination of valganciclovir hydrochloride in bulk and tablet dosage form. Validation study was performed to develop a simple, sensitive, rapid, accurate, and economical UV spectrophotometric method for the estimation of valganciclovir hydrochloride. UV 1800 double beam UV visible spectrophotometer with the pair of 10 mm path length matched Quartz cells were used for the study. Valganciclovir hydrochloride shows the maximum absorbance at 252 nm in distilled water. Beer’s law was obeyed at the concentration range of 1-6 µg/ml with the correlation coefficient of 0.9998. The regression equation was found to be Y=0.0379x-0.0039. The standard plot clearly shows a straight line passing through the origin. The percentage recoveries were found to be 99.99 to 100.72%. The percentage relative standard deviation was well within the range of <2%. The LOD and LOQ were found to be 0.1112 and 0.3372 µg/ml. The method was validated according to the ICH guidelines and it was applied successfully for the determination of valganciclovir hydrochloride tablets.
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