A simple, accurate and precise area under curve spectroscopic method was developed and validated for the estimation of dexamethasone in bulk and pharmaceutical dosage forms and AUC was measured at 236-246 nm in 0.1N HCl (hydrochloric acid). The linearity was found to be in the concentration range of 10-50 microgram/ml and the correlation coefficient was found to be 0.9992 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y =0.009 X + 0.007. The % recovery values were found to be within 98.74-100.8% showed that the method was accurate. The LOD and LOQ were found to be 0.101 and 0.304 respectively. The % RSD values were less than 2. The present methods were accomplishing the validation parameters according to ICH guidelines. The developed method was successfully applied for the quantitative estimation of dexamethasone in bulk and pharmaceutical dosage forms.
Loading....