A novel, precise, sensitive and accurate UV spectrophotometric method has been developed for the estimation of azathioprine in bulk and tablet dosage form. UV 1800 double beam UV visible spectrophotometer with the pair of 10 mm path length matched Quartz cells were used for the study. The azathioprine shows the maximum absorbance at 280 nm in distilled water. Beerâ??s law was obeyed at the concentration range of 1-6 mcg/ml with the correlation coefficient of 0.9994. A recovery study was found to be 98.8-100.49. The %RSD values are less the 2 in accordance to the ICH guidelines. The LOD and LOQ were found to be 0.0974 and 0.2953 respectively. This method was validated in terms of accuracy, precision and ruggedness in accordance to the ICH guidelines. This method applied for the estimation of azathioprine tablets.
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