Zero order derivative spectroscopic method was developed and validated for the estimation of dexamethasone in bulk and pharmaceutical dosage forms. The absorption maximum was at 241 nm in 1N hydrochloric acid. The linearity was found to be in the concentration range 10-50 µg/ml and the correlation coefficient was found to be 0.999 and it has showed good linearity, reproducibility, precision in this concentration range. The regression equation was found to be Y=0.008 X + 0.004. The % recovery values were found to be within 99.65-100.46% showed that the method was accurate. The LOD and LOQ were found to be 0.5544 and 1.6632 mcg/ml, respectively. The % RSD values were less than 2. The present method was accomplish the validation parameters according to ICH guidelines like accuracy, precision, linearity, range, ruggedness, limit of detection and limit of quantization. The developed method was successfully applied for the quantitative estimation of dexamethasone in bulk and pharmaceutical dosage forms.
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