Ivabradine hydrochloride is a first-in-class drug that is selective bradycardic agent with direct effect on the pacemaker If current of the sinoatrial node. It is used for the treatment of chronic coronary artery disease and chronic heart failure. The first-derivative UV-Spectrophotometric method is developed for the estimation of ivabradine hydrochloride in pure and pharmaceutical dosage form. The best selected solvent for derivatization is acetonitrile measuring the analytical response at 278 nm which is the units of distance from central zero baseline. The developed method is linear from 4-20 microgram/ml with coefficient of determination (r2) as 0.997. The Limit of detection and limit of quantification were found to be 1.216 microgram/ml and 3.685 microgram/ml respectively. The developed method is validated according to ICH guidelines. The proposed method is successfully applied for quantitation of Ivabradine Hydrochloride in pharmaceutical dosage form with good recovery and reproducibility. The proposed method is simple and cost effective.
Loading....