Embelia ribes fruits, which are commonly known as Vidanga, have long history of use in ayurvedic system of medicine in various forms like asava, aristha, lauha, and taila. Vidanga Churna is an ayurvedic formulation of Embelia ribes fruits and the active component in vidanga is embelin. One in-house batch of vidanga churna was prepared in laboratory and standardized according to WHO guidelines. Three different marketed formulations were procured from Ayurvedic medicine shop. Embelin was isolated from the in house churna and its identity was confirmed by physical data and spectroscopic studies. Different tests like determination of foreign matter, macroscopic and microscopic examination, determination of ash value, extractable matter, swelling index, foaming index and microorganisms were performed as per WHO guidelines and limits for the same were optimized. A UV spectrophotometric method was developed for the estimation of embelin in laboratory and marketed formulations. The content of embelin in raw material was found to be 2.2 % w/w. The developed method has been validated as per ICH guidelines. Developed spectrophotometric method shows good regression (0.9982) and the recovery of the embelin is in the range of 98.64-102.38 %. The limit of detection and limit of quantitation are found to be 0.05 µg/ml and 0.157 µg/ml, respectively. The results of the study revealed that the developed UV spectrophotometric method is simple, precise, and reliable for quantitative analysis, moreover, for the quality evaluation and control of vidanga.
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