A simple, novel, sensitive, and specific spectrophotometric method was developed and validated for the determination of Paracetamol and Tolperisone Hydrochloride in bulk and its combined dosage form. First order derivative spectroscopy method is adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges selected for evaluation. Paracetamol and tolperisone hydrochloride have λmax at 248nm and 255 nm respectively in methanol. The method was validated for linearity, accuracy and precision as pe ICH norms. The zero cross over point for paracetamol and tolperisone hydrochloride was 248.6nm and 255nm respectively.The LOD and LOQ value were found to be 0.48 and 1.58 ppm for paracetamol and 0.86 and 2.87 ppm for tolperisone hydrochloride respectively. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form.
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