A simple, reproducible and efficient reverse phase high performance liquid chromatography (RP-HPLC) method has been developed for estimation of rupatadine fumarate in its tablet dosage form. The separation was performed on a Hypersil BDS -C18 (250 mm×4.6 mm, 5 μm) column, the mobile phase was composed of 1% triethylamine (pH 3.0 using o-phosphoric acid)-acetonitrile (30∶70) and the flow rate was 1.0 mL•min-1 with UV detection at 252 nm and the retention time for rupatadine fumarate is 5 min. The linear dynamic response was found to be in the concentration of 50 μg-150 μg/ml. The correlation coefficient was found to be 0.9996. The %RSD of % recovery of rupatadine fumarate was found to be 0.899, 0.267, and 0.421 for 50%, 100% and 150% respectively. Proposed methods were found to be simple, accurate, precise and rapid and could be used for routine analysis. This condition is applied only for tablet dosage form. The statistical parameters and recovery studies were carried out and reported.was also checked by placebo blank and synthetic mixture analyses besides a recovery study via standard addition procedure.
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