A simple UV spectrophotometric method was developed and validated for quantitative determination of cinitapride and omeprazole, at their respective analytical wavelengths in fixed dose combination (FDCS) products. Method involve formation and solving of simultaneous equation by the measurement of absorbances at two wavelength 263.0 nm (max for cinitapride) and 302.0 nm (max for omeprazole). The different analytical performance parameters such as linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2 (R1) guidelines. Method obeyed the beer’s law and Calibration cures for both omeprazole and cinitapride were linear over concentration range of 2-10 µg/mL with correlation coefficients 0.9994 and 0.9998, respectively. The recovery of omeprazole and cinitapride was found to be in range of 98.15 to 101.92% and from 99.56 to 100.18%, respectively. The limit of determination for omeprazole and cinitapride was 0.09µg/ml and 0.03µg/ml, respectively. The limit of quantification for omeprazole and cinitapride was 0.29µg/ml and 0.11µg/ml, respectively. This developed method is simple, rapid, precise and accurate and was successfully employed for quantifying both drugs containing pharmaceutical formulations.
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