The present research work describes not only simple and rapid but also sensitive, accurate, precise, and reliable absorbance ratio (Q – ratio) method for the simultaneous estimation of Rabeprazole sodium and Levosulpiride in combined pharmaceutical formulation. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λmax of one of the two components. Rabeprazole sodium and Levosulpiride show an isoabsorptive point at 260.57 nm in methanol. The second wavelength used is 284 nm, which is the λmax of rabeprazole sodium in methanol. The linearity was obtained in the concentration range of 5 - 25 μg/ml for Rabeprazole sodium and 19 - 95 μg/ml Levosulpiride. The concentrations of both the drugs were determined by using ratio of absorbances at isoabsorptive point and at the λmax of Rabeprazole sodium. The method was successfully applied to pharmaceutical dosage form because no interference from the tablet excipients was found. The results of analysis have been validated statistically for linearity, LOD, LOQ, accuracy, precision, robustness and ruggedness of the proposed method. Present method is economical in the means of both time & cost.
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