A simple, sensitive, and precise high performance liquid chromatographic method has been developed for the simultaneous determination of esomeprazole magnesium (ESO) and domperidone (DOM) from combined capsule dosage form. The compounds were well separated on a ODS hypersil C-18 (4.6mm×250mm) column. The mobile phase consisting of (methanol: acetonitrile: 1% triethylamine solution = 60:30:10 v/v/v) at a flow rate of 0.8 mL/min with detection wavelength at 280 nm. The retention times for esomeprazole magnesium and domperidone were found to be 2.833 and 3.765 min, respectively .The linearity range and recovery amount were found to be 20-100 μg /ml and in between 99-101% respectively for both the drugs. The method was validated for specificity, linearity, accuracy, precision, LOD, LOQ. The method herein described can be employed for quality control and routine analysis of esomeprazole magnesium and domperidone in pharmaceutical dosage form.
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