Since methylcobalamin is the only form of vitamin B12 that can cross the blood brain barrier so it is also important in formulation .From literature survey it was found that there is no single method for the estimation of methylcobalamin in formulation containing multiple vitamin and minerals. Therefore a simple precise and accurate method was developed for the detection and estimation of methylcobalamin by RP-HPLC.The linearity value was found to be 0.9960. The % RSD for precision 0.8 ,0.4 &0.4 for retention time, area and amount. The accuracy studies were done for 80-120% sample and the results were 1.3,1.5 , 1.1 1.8 & 2.0 respectively. . The method passed the validation parameters and had the LOD & LOQ value as0.00195 %0.006519 respectively .
Loading....