A simple and sensitive HPTLC method has been developed for determination of Ambroxol and roxithromycin in its pharmaceutical dosage forms. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. Benzene: Chloroform: TEA (5:4:1v/v) as a mobile phase then spray with sulfuric acid and dry the plate at ahe room temperature. After that saturation time is optimized for the better separation The Rf value of Ambroxol HCl is 0.59±0.02 and of Roxithromycin is 0.39±0.02 were found. The linearity for both the drugs were in the range of 120-360 μg/ml and 300-900 μg/ml. The % recoveries of ambroxol hydrochloride and cetirizine hydrochloride were found to be in the range of 99.37-99.42 and 99.27-99.3, respectively. The proposed method was validated and successfully applied to the estimation of ambroxol hydrochloride and roxithromycin in combined dosage form.
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