A simple Reverse phase liquid chromatography method has been also developed and subsequently validated for the tablet dosage form. Here mobile phase consisting Methanol 25mM Triethylamine pH set to 3.6 with O-Phosphoric acid gave resolution of peaks and satisfied retention time in HPLC. C-18(250mm × 4.6mm i.d with particle size of 5 µm used with flow rate 0.6ml/min using UV detection at 254nm. The retention time of Amlodipine Besylate and Clopidogrel bisulphate were found at 4.6 and 6.2 respectively. In Which Linearity for AML and CLOP was found to be y = 6720x-16231, R2 = 0.998 in concentration range of 5-25µg/ml and 984.04+ 948780, R2 + 0.998 for 75-375µg/ml respectively. Recovery studies were found to be 99.16-100.5% for AML and 99.72-100.86% for CLOP. Other all the data (Precision, LOD and LOQ, Assay, Robustness) are within the specified criteria of ICH guideline. The proposed method was validated and successfully applied to the estimation of Amlodipine besylate and Clopidogrel bisulphate in combined dosage form.
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