The combination of Ofloxacin (OFL) and Cefpodoxime proxetil (CPD) is used in the treatment of upper and lower respiratory tract infection and typhoid fever. A simple, precise and accurate isocratic RP-HPLC method was developed and validated for determination of OFL and CPD in tablets. Isocratic RP-HPLC separation was achieved on a ACE C18 column (150 × 4.6 mm id, 5 µm particle size) using the mobile phase 0.2% triethyl amine in 20mM Ammoniun acetate buffer-Acetonitrile (65:35 v/v, pH 7.0) at a flow rate of 1.0 mL/min. The retention time of Ofloxacin and two isomer of Cefpodoxime Proxetil was 3.45, 9.95 and 11.15 min, respectively. The detection was performed at 272 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability and specificity. The method was linear in the concentration range of 2-20 µg/mL with a correlation coefficient of 0.9995 for both drugs. OFL and CPD were subjected to forced degradation studies. The degradation products obtained were well resolved from the pure drugs with significantly different Rt values. As the method could effectively separate the drugs from its degradation products, it can be used for stability-indicating analysis.
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