A reverse phase HPLC method is developed for the determination of Cinitapride and pantoprazole in pharmaceutical dosage forms. Chromatography was carried out on a C18 column [4.6 x 150mm, 5m, Make: 10AT SHIMADZU – SPD 10A Detector] using a mixture of sodium dihydrogen phosphate dihydrate buffer and acetonitrile (60:40 v/v) as the mobile phase at a flow rate of 1ml/min. Detection was carried out at 264 nm .The retention time of the drug Cinitapride and Pantoprazole was 2.51 min and 4.92 min. The method produced linear responses in the concentration range of 20 to 120μg/ml of Cinitapride and Pantoprazole. The LOD values for HPLC method for Cinitapride and Pantoprazole were found to be 0.17 and 0.61ng/ml. The LOQ for Cinitapride and Pantoprazole were foud to be 0.53 and 1.87 ng/ml respectively. The method was found to be applicable for determination of the drug in tablets.
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