Cefpodoxime proxetil (CPD) and Ambroxol hydrochloride (AMB) are used in the treatment of respiratory tract infection, chronic bronchitis, COPD. A simple, precise and accurate isocratic RP-HPLC method was developed and validated for determination of CPD and AMB in tablets. Isocratic RP-HPLC separation was achieved on a ACE C18 column (150 × 4.6 mm id, 5 µm particle size) using the mobile phase Acetronitrile: Phosphate buffer (pH 6.0): Methanol (25:35:40v/v) at a flow rate of 0.8 mL/min. The retention time of Cefpodoxime Proxetil and Ambroxol hydrochloride was 3.5 and 6.5 min. The detection was performed at 248 nm. The method was validated for linearity, precision, accuracy, robustness, solution stability and specificity. The method was linear in the concentration range of 5-50 µg/mL for CPD and 3-30 µg/mL for AMB with a correlation coefficient of 0.999 for both drugs. The intraday precision was 0.17-0.47% and 0.15-0.59% and for interday precision were 0.25-0.37% and 0.22-0.58% for CPD and AMB respectively. The accuracy (recovery) was found to be in the range of 99.09-99.85% and 99.46-99.98% for CPD and AMB respectively.
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