Aim of the present study was to perform preliminary phytochemical screening and evaluate the anti bacterial activity for three extracts ethanolic, petroleum ether and aqueous of Adhatoda vesica belonging to family Acanthaceae. Adhatoda vesica is an important herb in the Indian system of ayurvedic medicine. It has been traditionally used to treat cough, whooping cough, cold and clung phlegm in mouth, throat, chest or breast. It is also effective against Tuberculosis, fever, leukaemia and skin diseases. Study was conducted using aqueous, pet. Ether and ethanolic extract leaves. A preliminary phytochemical screening was also performed for qualitative determination of the phytoconstituents. Antibacterial assay was carried out using different strains of bacteria like Escherichia coli (MTCC No.40), Staphylococcus aureus (MTCC No.87), Proteus vulgaris (MTCC No.742), Pseudomonas aeruginosa (MTCC No.424), Bacillus subtilis (MTCC No.441), Staphylococcus epidermidis (MTCC No.9041) and Micrococcus luteus (MTCC No.106), using cup plate method. Phytochemical screening indicated the presence of phenols, tannins, alkaloids, anthraquinones, saponins, flavonoids, amino acids and reducing sugars. From anti microbial assay was found that ethanolic extract was effective against all the bacterial strains, where as mixed effect observed in remaining two extracts.
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