A complex three species model community fungi, was selected from organisms isolated from upper Lake of Bhopal. Growth rates of the species were determined in single and mixed batch culture growth. The rates were found to be significantly higher culture for Acremonium, Exophiala and Phialophora. All species attached to PVC in single and mixed culture to form biofilms Acremonium was significantly higher than attachment of the component species in mixed culture. Statistical analyses showed a significant difference in rate of colonization between single and mixed cultures for some species. Biofilm formation is major problem affecting Fresh water of Lake which causes major health problem hence biofilm formation further suggested that spores, not hyphae or vegetative cells, comprised the primary source of viable propagules.
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