Distilled cow urine commonly known as “Gau Mutra”, is a well-known medicament in Indian traditional medicine. On the basis of its traditional use and literature references, this plant was selected for evaluation of its antimicrobial activity against Staphylococcus aureus, Pseudomonas aureogenosa, Staphylococcus epidermis, Shigella flexineri, Bacillus substilis , E. Coli.& S.aureus,Bacillus subtilis, S.epidermidis using the agar well (cup plate) diffusion method. Distilled cow urine inhibited the growth of gram positive bacteria, with zone of inhibition between 7 - 10 mm, and a gram negative bacterium with zone of inhibition between 9-12mm. The activities of the distilled cow urine on the inhibited pathogens using the zone of inhibition were not as effective as the standard commercial antibacterial disks of tetracycline, Ofloxacin, Azithromycin, Piperacillin, Nitrofurantoin, Gentamicin, Cefotaxime, Norfloxacin.
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