Cathepsin L-like proteinases (CAL) are major digestive proteinases in the beetle Tenebrio molitor. Procathepsin Ls 2 (pCAL2) and 3 (pCAL3) were expressed as recombinant proteins in Escherichia coli. With the aim to find new agent against Cathepsin L-like proteinases, we have carried out target search in the zinc database and identified a target compound (zinc_200316000) using in silico docking analysis. Structure of the target protein was retrieved from protein data bank (pdb i.d.3qj3) and in silico analysis was performed on Autodock 4.2. The docking analysis revealed that ligand zinc_200316000 interacts with target protein via arene-cation and nonbonding interactions. The analysis divulges that present in silico docking results may be helpful for the optimization of the ligand for enhanced biological activity and future drug design.
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