Microbial keratitis is a major cause of monocular blindness in developing countries. Keratitis caused by bacteria, viruses, fungi and parasites. This study investigates that the universal primer designing the bacterial and fungal genes for clinical diagnosis of infectious eye samples by using C++ algorithms. Patients were examined with a slit lamp biomicroscope. The bacterial and fungal colonies were isolated in eye swabs of keratitis patients and identified the genes of isolated bacteria by using 16S rRNA and 18S rDNA gene type. The bacterial and fungal genes were sequenced and it deposited in the GenBank (NCBI). The primers were designed for those genes by using primer3 tool. The universal primer set (ie, forward and reverse primers) were designed for both bacterial and fungal gene primers by using C++ algorithm for finding all tandem repeats within the forward and reverse primers. The universal primer is used as a diagnostic kit for microbial diagnosis of keratitis cases in future. This study suggests that bioinformatics analyses are important to validate the choice of primers.
Loading....