This work presents a heterogeneous immunoassay using the integrated functionalities of\na channel and droplets in a digital microfluidic (DMF) platform. Droplet functionality in DMF\nallows for the programmable manipulation of discrete sample and reagent droplets in the range of\nnanoliters. Pressure-driven channels become advantageous over droplets when sample must be\nwashed, as the supernatant can be thoroughly removed in a convenient and rapid manner while the\nsample is immobilized. Herein, we demonstrate a magnetic bead-based, enzyme-linked\nimmunosorbent assay (ELISA) using..........
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