The aim of the present study was to prepare and evaluate niosomes using clopidogrel bisulphate as a model drug. The clopidogrel bisulphate niosomes were prepared by using span 60 with different proportions and cholesterol by two methods such as thin film hydration method and ether injection method. The prepared niosomes were characterized for drug entrapment efficiency, particle size, in-vitro studies and drug release kinetics. The preformulation studies such as solubility analysis, drug-excipients compatibility studies were performed. The niosomes of thin film hydration method, formulation TF-8 showed highest entrapment efficiency (80.1%) than the ether injection method formulation EF-8 showed little increase (46.55%). In-vitro release studies showed that the percentage amount of free drug release was 70.37% for TF-1 and 98.11% for EF-1 within 8 hrs. The microscopic examination of the prepared niosomes revealed spherical small unilamellar vesicles of 169.4, 170.3 and 175.4 for TF1, TF3 and TF6. The drug release kinetic data was best fitted for Koresmeyer-Peppa’s model exhibiting regression, r2 = 0.994 and showed good linearity. The clopidogrel bisulphate was successfully encapsulated into niosomes Span 60 (1:2:1) vesicles prepared by thin film hydration method showed highest entrapment efficiency and less % in-vitro drug release than the ether injection method.
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