The objective of the present investigation was to formulate and evaluate Biodegradable Gliclazide mucoadhesive microsphere using tamarind gum and Chitosan as polymers. Gliclazide microspheres were prepared by simple emulsification phase separation technique using glutaraldehyde as a cross-linking agent. Twenty preliminary trial batches, F1 to F20 batches of microspheres were prepared by using different volume of cross-linking agent, cross-linking time and 3:1 polymer-to-drug ratio. From these twenty batches of each polymer, the optimized formulation is selected based on the percentage of mucoadhesion, drug entrapment efficiency and sphericity of microspheres. A 32 full factorial design was employed to study the effect of independent variables, polymer-to-drug ratio(X1), and stirring speed(X2) on dependent variables percentage of mucoadhesion, drug entrapment efficiency, swelling index and in vitro drug release study. The drug polymer compatibility studies were carried out using FTIR and the stability studies were conducted for the optimized formulation. Among the two polymers, the best batch was tamarind gum exhibited a high drug entrapment efficiency of 69% and a swelling index 1.16 % mucoadhesive after 1hour was 70% and the drug release was also sustained for more than 10 hours. The polymer-to-drug ratio had a more significant effect on the dependent variables.
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